Phorbol diester receptor copurifies with protein kinase C.
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منابع مشابه
Phorbol diester receptor copurifies with protein kinase C.
The phorbol diester receptor present in the particulate fraction of rat brain was solubilized by divalent ion chelation in the absence of detergents. The soluble receptor was partially purified by (NH4)2SO4 precipitation, DEAE-cellulose, and gel filtration chromatography. The purified receptor required exogenous phospholipid for activity and displayed a Kd of 7 nM for [3H]phorbol 12, 13-dibutyr...
متن کاملFactors Influencing Chelator - stable , Detergent - extractable , Phorbol Diester - induced Membrane Association of Protein Kinase C
One of the early events a sociated with the treatment of cells by tumor promotor phorbol esters is the tight association of protein kinase C to the plasma membrane. To better understand the factors that regulate this process, phorbol ester-induced membrane binding of protein kinase C was studied using homogenates, as well as isolated membranes and purified enzyme. Addition of 12-O-tetradecanoyl...
متن کاملEvidence of protein kinase C involvement in phorbol diester-stimulated arachidonic acid release and prostaglandin synthesis.
Many stimulators of prostaglandin production are thought to activate the Ca2+- and phospholipid-dependent protein kinase first described by Nishizuka and his colleagues (Takai, Y., Kishimoto, A., Iwasa, Y., Kawahara, Y., Mori, T., and Nishizuka, Y. (1979) J. Biol. Chem. 254, 3692-3695. In this paper we report evidence that the activation of protein kinase C caused by 12-O-tetradecanoylphorbol-1...
متن کاملPhorbol Esters and Related Analogs Regulate the Subcellular Localization of b2-Chimaerin, a Non-protein Kinase C Phorbol Ester Receptor*
The novel phorbol ester receptor b2-chimaerin is a Rac-GAP protein possessing a single copy of the C1 domain, a 50-amino acid motif initially identified in protein kinase C (PKC) isozymes that is involved in phorbol ester and diacylglycerol binding. We have previously shown that, like PKCs, b2-chimaerin binds phorbol esters with high affinity in a phospholipid-dependent manner (Caloca, M. J., F...
متن کاملHeterogeneous localization of protein kinase C in rat brain: autoradiographic analysis of phorbol ester receptor binding.
Protein kinase C is a calcium- and phospholipid-stimulated enzyme present in high concentration in the brain. Phorbol esters are potent tumor promoters that bind to specific receptors with high affinity. Several lines of evidence indicate that the phorbol ester receptor is identical to protein kinase C. To determine the distribution of protein kinase C, we have localized phorbol ester receptors...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1983
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.80.1.36